New vectors for epitope tagging and gene disruption inSchizosaccharomyces pombe
نویسندگان
چکیده
منابع مشابه
New vectors for epitope tagging and gene disruption in Schizosaccharomyces pombe.
We describe a series of new vectors for PCR-based epitope tagging and gene disruption in the fission yeast Schizosaccharomyces pombe, an exceptional model organism for the study of cellular processes. The vectors are designed for amplification of gene-targeting DNA cassettes and integration into specific genetic loci, allowing expression of proteins fused to 12 tandem copies of the Pk (V5) epit...
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We have examined the feasibility and efficiency of PCR-mediated direct gene disruptions in the fission yeast Schizosaccharomyces pombe. In the present study, the S.pombe ura4+ gene was amplified by PCR with oligonucleotides that had short flanking regions ( approximately 40 bp) to the target gene. Using this purified PCR product we were able to disrupt genes in an S. pombe strain bearing aura4 ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2013
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000114100